Sugar Substitute Analysis

High-performance anion-exchange chromatography with pulsed amperometry detection. This technique enables the selective separation of sugar alcohols in an alkaline medium without derivatization. The electrochemical detector responds to hydroxyl-bearing compounds, providing high selectivity even in complex food matrices. Typical quantification range: 0.1–20 g/100 g, depending on the matrix.

HPLC with refractive index detection.
It is widely used for polyol determination in simpler matrices (e.g. confectionery and beverages). It is less selective than HPAEC-PAD in complex matrices containing multiple carbohydrate species but is well-suited to routine screening and quality control applications.

Multi-component determination of acesulfame K, aspartame, saccharin, sucralose, and cyclamate in a single chromatographic run. Diode array detection provides spectral confirmation. This is the standard method for most food categories where sweetener concentrations are at or above mg/kg levels.

Tandem mass spectrometry for unambiguous identification and trace-level quantification. Required for steviol glycosides (including differentiation of rebaudioside A, D and M), advantame and neotame — substances for which UV detection lacks sufficient selectivity or sensitivity. It is also used for screening undeclared sweeteners in 'clean label' products.