Skip to content

Vitamin Analysis

Accredited quantification of all vitamins in food, feed, supplements and pharmaceuticals via HPLC, LC-MS/MS and validated microbiological assays (ISO/IEC 17025). 

Request Analysis
Contact Us
A person holding a pipette next to test tubes in a laboratory setting.

From Bioassay to Mass Spectrometry

A variety of heart-healthy foods, including fruits, vegetables, and nuts, rich in essential vitamins and nutrients.

Accurate vitamin quantification is essential for regulatory compliance, label accuracy and product development. We measure a broad spectrum of vitamins in food, feed, supplements and pharmaceuticals using validated analytical approaches, including HPLC-DAD, HPLC-UV, LC-MS/MS and VitaFast® microbiological assays. These methods enable both targeted quantification and the determination of total biologically active vitamin content.

Depending on the analyte and matrix, multiple analytical methods may be applied to ensure accuracy and robustness of results. This comprehensive capability supports reliable data generation across simple and complex matrices — from raw materials to finished products.

Testing Portfolio

Quantification of all vitamins in native and fortified form – including provitamins and relevant vitamer forms.
Select a group to view full parameter lists and method details.

Analytical Methods

Two complementary platforms – each optimised for specific vitamin classes, matrices and regulatory requirements.
Instrumental

HPLC & LC-MS/MS

High-performance liquid chromatography with DAD, FLD or tandem mass spectrometry enables analyte-specific quantification of individual vitamin forms.

LC-MS/MS provides unambiguous identification and quantification at trace levels – essential for native vitamin D₃, K isomer differentiation (MK-4/MK-7), biotin and folate in complex or low-concentration matrices.

Proprietary Technology

VitaFast® Microbiological Assay

VitaFast® adapts classical microbiological vitamin determination to a ready-to-use 96-well microplate format. The assay quantifies total biologically active vitamin content based on vitamin-dependent microbial growth – including free, bound and matrix-entrapped forms that instrumental methods may underestimate.

Explore Kit Production

Matrices We Analyse

Methods validated across all major product types; matrix-specific method availability and LOQs depend on analyte and sample composition.

Regulatory Framework

Vitamin declarations and compositional requirements are governed by a set of EU and national regulations. Depending on whether vitamins are added to foods, declared on labels, used in supplements or included in feed premixes, analytical results must comply with the specific framework applicable to each product category.

All vitamin testing at ifplabs is performed under ISO/IEC 17025 accreditation.

Talk to Our Experts

Regulation (EU) No 1169/2011
Rules for food information and nutrition labelling; vitamins must be declared when added or when a claim is made.

Regulation (EC) 1925/2006 (Fortification)
Harmonised rules for adding vitamins and minerals to foods, including permitted nutrient forms (Annex I–II).

Directive 2002/46/EC (Food Supplements)
Framework for vitamin-containing supplements; permitted vitamin/mineral sources listed in Annex I–II.

Regulation (EC) 767/2009 (Feed)
Labelling and composition rules for feed, including declaration of added vitamins in compound feed and premixes.

UK (GB) Retained EU Law
Former EU nutrition and supplement legislation retained and adapted in Great Britain; EU rules continue to apply in Northern Ireland.

Pharmacopoeial Standards (Ph. Eur., USP)
Reference methods for vitamin testing in medicinal products and raw materials.

Related Analytics

Healthy food concept, Hand of nutritionist doctor pointing fruit.

Nutrition

Proximate analysis, energy calculation, fatty acid profiles, sugars, fibre and mineral content for full nutritional declarations per EU FIC or UK requirements.

Explore Nutrition
Xanthan Gum Spilled from a Teaspoon.

Additives

Ascorbic acid (E 300) serves dual functions as antioxidant additive and vitamin C. Where both roles are relevant, analysis can be combined to avoid duplicate testing.

Explore Additives