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Mycotoxin Analysis

Quantitative determination of all EU-regulated mycotoxins in food, feed, spices, nuts, cereals and cocoa with express turnaround options.

Sunlit wheat spikelets in a field captured in a panoramic view.

From Regulated Limits to Trace-Level Quantification – Mycotoxins in the Food and Feed Chain

A close-up of corn kernels, highlighting their importance as a staple cereal grain for many cultures worldwide.

Mycotoxins are secondary metabolites produced by Aspergillus, Penicillium, Fusarium and Alternaria. They can contaminate crops pre- and post-harvest and remain stable through processing. Many exhibit hepatotoxic, nephrotoxic, immunotoxic or carcinogenic effects; aflatoxin B₁ is classified as IARC Group 1.

We quantify all regulated and extended mycotoxins under ISO/IEC 17025 – aflatoxins and ochratoxin A by HPLC-FLD, Fusarium toxins by LC-MS/MS.

Parameter Portfolio

All EU-regulated mycotoxins plus extended markers – grouped by producing organism and toxicological relevance.

Aspergillus Toxins

Aflatoxins and ochratoxin A – primarily associated with nuts, oilseeds, spices, cereals, cocoa, coffee and dried fruits. Includes aflatoxin M1 for dairy products.

Methods: HPLC-FLD.

Common analytes: Aflatoxins B₁ + B₂ + G₁ + G₂, aflatoxin B₁ (single determination), aflatoxin M₁, ochratoxin A (OTA).

Fusarium Toxins

Trichothecenes, zearalenone and fumonisins – primarily in cereals such as wheat, maize and barley. Regulated maximum levels for T-2/HT-2 apply EU-wide; deoxynivalenol remains the most prevalent cereal toxin.

Methods: LC-MS/MS.

Common analytes: Deoxynivalenol (DON), zearalenone (ZEA), T-2/HT-2 (sum), nivalenol (NIV), 3-acetyl-DON / 15-acetyl-DON, diacetoxyscirpenol (DAS), fusarenone X, fumonisins B₁ + B₂.

Analytical Methods

Two complementary platforms – each optimised for different mycotoxin classes, matrices and sensitivity requirements.
Reference Method

HPLC-FLD

High-performance liquid chromatography with fluorescence detection for aflatoxins (B₁, B₂, G₁, G₂), aflatoxin M₁ and ochratoxin A. Immunoaffinity clean-up ensures high specificity and consistent quantification at regulatory levels.

Suitable for: Aflatoxins (B‑, G-series, M1), OTA.

Multi-Mycotoxin Quantification

LC‑MS/MS

Liquid chromatography with tandem mass spectrometry for simultaneous quantification of Fusarium toxins and modified forms. A single extraction covers the full trichothecene and fumonisin range without separate clean-up per analyte.

Suitable for: Trichothecenes (DON, ZEA, T-2/HT-2), fumonisins, modified (masked) forms.

Analytical Interpretation

Key analytical considerations that influence how mycotoxin results are evaluated and interpreted across different matrices.
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Cereals & Bakery Products

Cereals are highly susceptible to Fusarium toxins due to field infection and moisture at harvest. Processing reduces but does not eliminate toxin levels.

Typical applications: Raw-material checks, incoming goods control, baby-food compliance, flour and milling monitoring.

A still life of nuts, legumes, spices, raisins, and coffee arranged in bulk in vertical lines.

Nuts, Oilseeds & Dried Fruits

Warm storage conditions and long supply chains favour Aspergillus contamination; lots often show strong heterogeneity.

Typical applications: Export testing, high-risk batch screening, storage monitoring, release testing for nut and dried-fruit processors.

A top view of spice bottles arranged in a patterned composition.

Herbs & Spices

Tropical origin, open-air drying and fine grinding increase aflatoxin and OTA risk and often produce uneven contamination profiles.

Typical applications: Supplier verification, import controls, blending checks, retail compliance programmes.

A close-up full-frame view of whole cocoa beans.

Cocoa & Coffee

Fermentation, drying and long storage influence OTA formation; roasting has limited effect on toxin stability.

Typical applications: Origin comparison, batch release, QC of cocoa powders and coffee beans.

Milk powder in spoon with can and baby bottle milk in bowl on wooden table.

Milk & Dairy Products

Aflatoxin M1 reflects the aflatoxin burden of dairy feed; seasonal and feeding-related variations are common.

Typical applications: Milk surveillance, dairy processing checks,  aflatoxin M₁ regulatory compliance.

A pile of beetroot pellets as a renewable animal feed source on a white background.

Animal Feed

Feed combines field- and storage-related risks; heterogeneous mixtures require representative sampling for reliable assessment.

Typical applications: Feed-mill monitoring, livestock risk assessment, QA programmes, compliance with Directive 2002/32/EC.

Illustration Law

Regulatory Framework

Regulated under Regulation (EU) 2023/915 defining maximum levels for mycotoxins in food, and under Directive 2002/32/EC for undesirable substances in animal feed. Matrix-specific limits and processing effects play a key role in result interpretation.

UK market: EU-derived requirements for mycotoxins apply under UK retained EU law, with assessment against the applicable UK legislation.

Related Analytics

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Phytochemicals

Pyrrolizidine alkaloids, tropane alkaloids, opium alkaloids, coumarin – plant-derived contaminants often co-occurring with mycotoxins in botanical matrices.

Explore Phytochemicals
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Residues

Multi-screening of ~700 pesticides. Fungicide residues (e.g. azoles) are often tested alongside mycotoxins for comprehensive crop quality assessment.

Explore Residues